rabbit anti glucagon  (Bio-Rad)

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Expression of 3 hub genes (A) Gene expression of KCNK1, PCDH1 and SDR16C5. (B) Expression of KCNK1 in a single cell. (C) Expression of PCDH1 in a single cell. (D) Expression of SDR16C5 in a single cell.

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Expressing, Gene Expression

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Related assays on the expression of hub genes. (A–C) mRNA expression of KCNK1, PCDH1 and SDR16C5 in HPNE and BXPC3. (D) mRNA expression of PCDH1 in HPNE and multiple PDAC cell lines. (E) Matched pairs of PDAC tissues and adjacent non-neoplastic pancreatic tissues were stained with PCDH1-specific antibodies for immunohistochemical analysis. Representative images of tissues and tumor-infiltrating cells are shown (100×, scale bar = 100 μm; 400×, scale bar = 20 μm). The MOD of PCDH1 is obtained by analyzing the photo-optical density with IPP software. (F) ROC curve of PCDH1 in PDAC. (G) Protein-protein interaction network of PCDH1. (ns: P >0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Expressing, Staining, Immunohistochemical staining, Software

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Correlation between PCDH1 and immune infiltration. (A) Correlation between PCDH1 and immune score. (B) Correlation between PCDH1 and stromal score. (C) Correlation between PCDH1 and ESTIMATE score. (D) Correlation between PCDH1 and immune cells. (* P < 0.05, ** P < 0.01).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques:

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Chemosensitivity of PCDH1. (A) Correlation between GDSC drug sensitivity and PCDH1 mRNA expression (B) Correlation between CTRP drug sensitivity and PCDH1 mRNA expression.

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Knockdown of PCDH1 inhibited the proliferation of PDAC. (A) Efficiency of knocking down mRNA of PCDH1 si-RNA in BXPC3. (B) Efficiency of knocking down mRNA of PCDH1 si-RNA in SW1990. (C, E) MTT assays reveal the proliferation ability of control group and PCDH1 knockdown group in cell line BXPC3 and SW1990. (D, F) Colony formation assays reveal the proliferation ability of control group and PCDH1 knockdown group in cell line BXPC3 and SW1990. (ns: P >0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Knockdown, Control

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Knockdown of PCDH1 inhibited the migration of PDAC. (A) Transwell assays show the migration ability of the control group and the PCDH1 knockdown group in the BXPC3 cell line. (Scale bar = 50μm) (B) Transwell assays show the migration ability of the control group and the PCDH1 knockdown group in the SW1990 cell line. (Scale bar = 50μm) (C) Wound healing assays exhibit the migration ability of the control group and the PCDH1 knockdown group in the BXPC3 cell line. (Scale bar = 100μm) (D) Wound healing assays exhibit the migration ability of the control group and the PCDH1 knockdown group in the SW1990 cell line. (Scale bar = 100μm) (ns: P >0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Knockdown, Migration, Control

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Knockdown of PCDH1 inhibited the tumor stemness of PDAC cell lines. (A) Relative expression of CD24 mRNA in the control group and the PCDH1 knockdown group. (B) Relative expression of CD133 mRNA in the control group and the PCDH1 knockdown group. (C) Detection of CD24 + cells in the control and PCDH1 knockdown groups via flow cytometry in BXPC3 and SW1990 cell lines. (D) Detection of CD133 + cells in the control and PCDH1 knockdown groups via flow cytometry in BXPC3 and SW1990 cell lines. (E, F) Percentage of CD24 + cells between the control group and the PCDH1 knockdown group (G, H) Percentage of CD133 + cells between the control group and the PCDH1 knockdown group. (I, J) Sphere formation assay was performed to detect sphere formation ability of the control group and the PCDH1 knockdown group in BXPC3 and SW1990. (K, L) Diameter of tumor spheres at 24 h, 48 h and 5 days in the control group and the PCDH1 knockdown group.(Scale bar = 500μm) (ns: P >0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Knockdown, Expressing, Control, Flow Cytometry, Tube Formation Assay

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: PCDH1 promotes proliferation and migration of PDAC through activation of PI3K/Akt pathway. (A) Heatmap of differential expression genes between the NC and si-PCDH1 groups. (B) KEGG enrichment analysis demonstrates pathways associated with differential expression genes. (C, D) Expression levels of mRNA for six molecules in the PI3K/Akt pathway were detected by qRT-PCR. (*** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Migration, Activation Assay, Quantitative Proteomics, Expressing, Quantitative RT-PCR

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: PCDH1 promotes proliferation and migration of PDAC through activation of PI3K/Akt pathway. (A, B) MTT assay was used to detect the proliferation ability of the control, IGF-1, si-PCDH1, and si-PCDH1 + IGF-1 groups. (C–E) A colony formation assay was performed to detect the proliferation ability of the four groups. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Migration, Activation Assay, MTT Assay, Control, Colony Assay

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: PCDH1 promotes proliferation and migration of PDAC through activation of PI3K/Akt pathway. (A) Transwell assays present the migration capacity of the control, IGF-1, si-PCDH1, and si-PCDH1 + IGF-1 groups. (Scale bar = 100μm) (B, C) Counts of cell migration in four groups. (D, F) Wound healing assays exhibit the migration ability of the four groups. (Scale bar = 100μm) (E, G) Rate of cell migration in four groups. (** P < 0.01, *** P < 0.001, **** P < 0.00011).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Migration, Activation Assay, Control